*Cultivation and high-resolution microscopy of cells
*Immunofluorescence staining and fluorescence microscopy of living and fixed cells
*Live cell imaging over extended time periods
*Transfection assays
*Differential interference contrast (DIC) microscopy when used with a DIC lid
Want to know if you should use a glass or a polymer bottom for your application? Find out here.
Specifications
Outer dimensions (w x l) | 25.5 x 75.5 mm2 |
Number of wells | 18 |
Dimensions of wells (w x l x h) | 5.7 x 6.1 x 6.8 mm3 |
Volume per well | 100 μl |
Height with/without lid | 8.2/6.8 mm |
Growth area per well | 0.34 cm2 |
Coating area per well | 1.15 cm2 |
Bottom: ibidi Polymer Coverslip | |
Technical Drawing
Technical drawings and details are available in the Instructions (PDF).
*Chambered coverslip with 18 independent wells and a non-removable polymer coverslip-bottom
*ibiTreat (tissue culture-treated) surface for optimal cell adhesion
*Imaging chamber slide with excellent optical quality for high-end microscopy
*Closely fitting lid for low evaporation
*Individual well walls for minimizing well-to-well crosstalk and contaminations
*Compatible with staining and fixation solutions
*Biocompatible plastic material—no glue, no leaking
*Also available as an adhesive version without a bottom: sticky-Slide 18 Well
*Also available with a Glass Coverslip Bottom: μ-Slide 18 Well Glass Bottom for special microscopic applications
The Principle of the μ-Slide 18 Well
The Coverslip Bottom
The μ-Slide 18 Well comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 18 Well without any bottom, or and as the μ-Slide 18 Well Glass Bottom for special microscopic applications.
Find more information and technical details about the coverslip bottom of the ibidi chambers here.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
Find more information about the different surfaces of the ibidi chambers here.
Multiple conditions on one μ-Slide: For immunofluorescence stainings, toxicological screenings, cell surface coatings.
Fluorescence microscopy of human endothelial cells (HUVEC) in a μ-Slide 18 Well ibiTreat. Red: alpha-tubulin, green: F-actin, stained with LifeAct-TagGFP2 Protein; blue: nuclei (ibidi Mounting Medium with DAPI). 60x objective lens, oil immersion.
